Journal: Nature protocols
Article Title: Directed differentiation of ureteric bud and collecting duct organoids from human pluripotent stem cells
doi: 10.1038/s41596-023-00847-2
Figure Lengend Snippet: Antibodies
Article Snippet: Section concentration Whole-mount concentration RRID GATA3 Cell Signaling Technologies 5852S 1:300 1:300 AB_10835690 PAX2 R&D systems AF3364 1:200 1:200 AB_10889828 LHX1 Abcam ab229474 1:200 1:200 AB_2924798 CDH1 BD systems 610181 1:500 1:500 AB_397580 SOX9 Novusbio NBP1-85551 1:200 1:200 AB_11002706 RET R&D Systems AF1485 1:200 1:200 AB_354820 AQP2 Millipore 178612 1:200 Not tested AB_2056864 Donkey anti-goat AF488 Jackson Immuno 705-545-147 1:500 1:500 AB_2336933 Donkey anti-goat AF594 Jackson Immuno 705-585-147 1:500 1:500 AB_2340433 Donkey anti-rabbit AF488 Jackson Immuno 711-545-152 1:500 1:500 AB_2313584 Donkey anti-rabbit AF594 Jackson Immuno 711-585-152 1:500 1:500 AB_2340621 Donkey anti-rabbit AF647 Jackson Immuno 711-605-152 1:500 1:500 AB_2492288 Donkey anti-mouse AF594 Jackson Immuno 715-585-151 1:500 1:500 AB_2340855 Donkey anti-mouse AF647 Jackson Immuno 715-605-151 1:500 1:500 AB_2340863 Open in a separate window Antibodies. . Other reagents 1× PBS, pH 7.4 (Thermo Scientific, cat. no. 10010023).
Techniques: Concentration Assay